"I have made the statement that I'm going to make on this," she told reporters at a Capitol Hill news conference. "I don't have anything more to say about it. I stand by my comment."
But Republicans aren't letting this one slide.
Ken Spain, spokesman for the National Republican Congressional Committee, issued a statement after the news conference calling Pelosi a political liability to the Democratic party.
"Her obsession with the previous administration and her disdain for America's intelligence officials has reduced her to cheerleader status within the far left wing of her party and a distraction to the substantive debate over how to best move our economy forward," said Spain.
Earlier this month, Pelosi told reporters that she had not been told that waterboarding had been used against terrorism suspects, even though it had been. When asked whether she was accusing the CIA of lying to her, she said "yes."
Pelosi has asked the CIA to declassify information supporting her claims. The CIA sent lawmakers its notes and memos on 40 congressional briefings on the interrogation techniques. But that document has been found to include several errors, leaving in question exactly what Pelosi was told.
The GOP has seized on her accusation that the CIA misled Congress, contending that the California Democrat's remarks have demoralized the intelligence community. House Republicans on Thursday demanded that a bipartisan panel investigate her allegations.
"To have this charge out there and not have it resolved I think is damaging to our intelligence efforts, and certainly will have a chilling effect on our intelligence professionals around the world," said House Republican leader John Boehner, R-Ohio.
Democrats beat back the proposal, calling it a political ploy. Republicans Ron Paul of Texas and Walter Jones of North Carolina joined Democrats in a 252-172 vote to block the resolution.
Upon leaving the news conference on Friday, Pelosi declined to answer a question about whether she had called CIA Director Leon Panetta to discuss the matter further.
Instead, Pelosi had stuck faithfully to her script, saying Democrats were making progress on other issues.
"We're going forward in a bipartisan way for jobs, health care, energy for our country," she said. Regarding the CIA's briefing of Congress on waterboarding, "I won't have anything more to say about it."